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Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation BABT
Tychanowicz,Giovana Kirst; Souza,Daniela F. de; Souza,Cristina G. M.; Kadowaki,Marina Kimiko; Peralta,Rosane Marina.
Pleurotus pulmonarius (Fr) Quélet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper; Laccase; Pleurotus pulmonarius; Phenoloxidase; Solid state fermentation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600002
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High levels of β-xylosidase in Thermomyces lanuginosus: potential use for saccharification BJM
Corrêa,Juliana Moço; Christi,Divair; Torre,Carla Lieko Della; Henn,Caroline; Conceição-Silva,José Luis da; Kadowaki,Marina Kimiko; Simão,Rita de Cássia Garcia.
ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high β-xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of β-xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce β-xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Experimental design; Saccharification; Β-Xylosidase; Thermomyces lanuginosus; Atlantic Forest.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300680
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